Protein Analysis Services

Transcription factors (TF) regulate gene transcription through DNA-protein interactions, such as direct binding with DNA. In order to mediate a specific gene transcription, TFs bind in a sequence-specific manner to certain promoter sequences that are usually located upstream from its target gene. By combining chromatin immunoprecipitation using TF specific antibodies, also known as the ChIP assay, then labeling IP-captured promoter-DNA as a probe for hybridization with an array that is pre-spotted with various promoter sequences, it is possible to determine many genetic targets for a given TF. The first generation technology called "ChIP-on-chip" has demonstrated its effectiveness (Ren, B. et al., (2000) Science 290:2306-2309).