Life Sciences

CapitalBio HybSet™ microarray hybridization cassette is designed to hold a 25 mm × 75 mm microarray slide in an isolated environment to prevent evaporation of the hybridization solution, thus reduce variation of the results and improve the S/N ratio of the hybridization image. Each HybSet™ consists of a base-board, two brackets, a sealing ring (gasket), a cover board, and a pair of metal retaining clips. The sealing ring and metal clips ensure that the hybridization chamber remains closed and watertight in the waterbath, guaranteeing uniform temperature and humidity. The cassette can apply to water bath or air bath. A series of brackets can be chosen alternatively to adapt to one standard slide or one slide with the SmartGrid™ gasket or one slide with the SmartGrid™ and SmartCover.

The Human Genome is composed of 3 billion nucleotides carrying hereditary information which determines an individual´s genetic potential. There is about 0.1% - 0.2% genomic DNA sequence difference between races and individuals, due mainly to single nucleotide polymorphisms (SNPs). Many of the SNPs can generate different inherited biochemical characteristics. For example, the ABO blood group site markers, leukocyte HLA site markers and individual disparities in drug metabolism. Knowledge of the DNA sequence differences and single nucleotide polymorphisms and their significance has begun to revolutionize diagnosis, treatment, prognosis and prevention.
 

Genomic DNA gains and losses can play a causal role in many diseases. Comparative genomic hybridization (CGH) is a powerful technique for the detection of chromosomal imbalances. CGH can also be used to identify different genes in diverse subtypes of the same species, thus indirectly confirming those phenotypes. The CGH method is based on a comparison of the test and control DNA samples which are labeled with different fluorophores, equally mixed, and simultaneously hybridized on one microarray, which can measure the DNA copy number differences between the test and control samples rapidly .
 

CapitalBio pre-spotted expression profiling arrays consist of various high quality cDNA and Oligo arrays, such as human, mouse, rice, cotton, silkworm genomic expression arrays, and some specific classes of functional expression arrays. These preprinted arrays have been widely used in life science research for genotyping or gene expression detection of target biological samples. Genomic array studies permit total analysis of cell genomic expression profiles, discovery of differential genes or differentially expressed genes, and discovery of cell phenotype and drug-effect related genes. Classified functional genomic arrays are useful for repeated and detailed experimental analysis for specific functionally-related genes.
 

Description
CapitalBio´s Expression Profiling Array Services guarantee the quality of your results. The exceptional quality of our services were highlighted in the recent FDA-sponsored MicroArray Quality Control (MAQC) project (Nature Biotechnology,24(9): 1140-1150, 2006). CapitalBio’s highly trained specialists manufacture, hybridize, scan, and conduct a preliminary analysis of your arrays. There is no need for you to train your lab personnel in highly specialized techniques or purchase expensive instruments to fabricate arrays in-house. Moreover, because CapitalBio´s products range from ready-to-use arrays to customizable array designs, you will be able to explore the complex bio-world and answer your research questions more quickly and cost effectively.

CapitalBio cRNA Amplification and Labeling Kit uses an improved RNA amplification protocol. The procedure makes use of an in vitro transcription (IVT) technique using PolyA at the 3´ end of mRNA to produce higher yields of cRNA. Labeled cDNA molecules are generated by reverse transcription and by subsequent Klenow Fragment Polymerase labeling.

The CapitalBio Real-Time qPCR Universal Kits provide all the necessary components to perform quantitative Real-Time PCR using EvaGreen or TaqMan probe for detection. The kit can meet various test requirements and can be used with a wide variety of instruments with higher sensitivity and reproducibility.